2YMO

Crystal structure of Pf12 tandem 6-cys domains from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	41.660, 76.870, 85.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.500 - 1.900
R-factor	0.2424
Rwork	0.240
R-free	0.28848
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2loe
RMSD bond length	0.011
RMSD bond angle	1.295
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.520	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.510
Number of reflections	22181
<I/σ(I)>	16.1	3.8
Completeness [%]	99.8	99.7
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20 MM CALCIUM CHLORIDE DIHYDRATE, 20 MM CADMIUM CHLORIDE HYDRATE, 20 MM COBALT CHLORIDE HEXAHYDRATE AND 20% PEG 3350