2YLN
Crystal structure of the L-cystine solute receptor of Neisseria gonorrhoeae in the closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.730, 63.430, 44.650 |
| Unit cell angles | 90.00, 96.89, 90.00 |
Refinement procedure
| Resolution | 30.960 - 1.120 |
| R-factor | 0.11087 |
| Rwork | 0.109 |
| R-free | 0.14176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zsf |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.137 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.960 | 1.150 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 75069 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 99.0 | 90.7 |
| Redundancy | 3.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 1.6 M AMMONIUM SULFATE, 100 MM NA-CITRATE PH 4.0. |
