2YLK
Carbohydrate-binding module CBM3b from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 95.456, 95.456, 82.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.689 - 2.200 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.228 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.540 |
| Number of reflections | 37875 | |
| <I/σ(I)> | 17.3 | 1.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.97 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20%(V/V) PEG 3350 0.2 M DIAMMONIUM HYDROGEN CITRATE., pH 7.5 |
