2YLH

Structure of N-terminal domain of Candida albicans Als9-2 G299W mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-16
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	35.200, 68.700, 121.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.050 - 1.700
R-factor	0.20772
Rwork	0.206
R-free	0.23664
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y7n
RMSD bond length	0.022
RMSD bond angle	1.622
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.050	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.280
Number of reflections	32813
<I/σ(I)>	17.4	5.88
Completeness [%]	97.8	94.4
Redundancy	5.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1