2YLC
Structure of Salmonella typhimurium Hfq in complex with U6 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2010-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 6 |
| Unit cell lengths | 61.190, 61.190, 28.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.595 - 1.300 |
| R-factor | 0.1342 |
| Rwork | 0.132 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ylb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 15112 | |
| <I/σ(I)> | 16.6 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M NASCN, 20% PEG3350, pH 8.0 |
