2YLC
Structure of Salmonella typhimurium Hfq in complex with U6 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2010-06-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 6 |
Unit cell lengths | 61.190, 61.190, 28.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.595 - 1.300 |
R-factor | 0.1342 |
Rwork | 0.132 |
R-free | 0.18160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ylb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.600 | 1.400 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 15112 | |
<I/σ(I)> | 16.6 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.1 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.2 M NASCN, 20% PEG3350, pH 8.0 |