2YL7
CYTOCHROME C PRIME FROM ALCALIGENES XYLOSOXIDANS: AS ISOLATED L16G VARIANT AT 0.9 A RESOLUTION - RESTRAINT REFINEMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.377, 53.377, 181.652 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.230 - 0.900 |
| R-factor | 0.12478 |
| Rwork | 0.124 |
| R-free | 0.13960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 115947 | |
| <I/σ(I)> | 33 | 3.3 |
| Completeness [%] | 98.3 | 83 |
| Redundancy | 9.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS PH 7.5 |
