2YL0
CYTOCHROME C PRIME FROM ALCALIGENES XYLOSOXIDANS: AS ISOLATED L16A VARIANT AT 0.95 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0,8 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.400, 53.400, 181.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.220 - 0.950 |
| R-factor | 0.13284 |
| Rwork | 0.132 |
| R-free | 0.14968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.000 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmerge | 0.060 | 0.990 |
| Number of reflections | 103306 | |
| <I/σ(I)> | 13.6 | 1.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, TRIS PH 7.5 |
