2YKT
Crystal structure of the I-BAR domain of IRSp53 (BAIAP2) in complex with an EHEC derived Tir peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-21 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.354, 187.362, 36.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.440 - 2.110 |
R-factor | 0.23559 |
Rwork | 0.233 |
R-free | 0.27604 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y2o |
RMSD bond length | 0.022 |
RMSD bond angle | 1.901 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.440 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.080 | 0.530 |
Number of reflections | 18190 | |
<I/σ(I)> | 20.13 | 3.13 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 16% (W/V) PEG 3350, 0.3M AMMONIUM SULPHATE |