2YKF
Sensor region of a sensor histidine kinase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9190, 0.9187, 0.9050 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.590, 81.270, 105.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 2.000 |
| R-factor | 0.19021 |
| Rwork | 0.188 |
| R-free | 0.23956 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.927 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.550 |
| Number of reflections | 21659 | |
| <I/σ(I)> | 16.5 | 3.5 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 | ||
| 1 | 7.5 | pH 7.5 |
