Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-18 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.930, 110.880, 60.930 |
| Unit cell angles | 90.00, 131.20, 90.00 |
Refinement procedure
| Resolution | 55.810 - 1.550 |
| R-factor | 0.20779 |
| Rwork | 0.206 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ycc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 60049 | |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 97.6 | 100 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 2.45 M (NH4)2 SO4, 0.1 M HEPES (PH 6.5). |
