2YJY
A specific and modular binding code for cytosine recognition in PUF domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-03-12 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.796, 59.340, 344.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.977 - 2.598 |
| R-factor | 0.2153 |
| Rwork | 0.210 |
| R-free | 0.30560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m8y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.980 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.270 |
| Number of reflections | 20661 | |
| <I/σ(I)> | 9 | 3.4 |
| Completeness [%] | 86.7 | 79.2 |
| Redundancy | 3.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 30% PEG 3350, 0.2M SODIUM TARTRATE, 0.1M BIS-TRIS PH 6.0 |
