2YJT

Crystal structure of E. coli DEAD-box protein SrmB bound to regulator of ribonuclease activity A (RraA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-29
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	73.390, 73.390, 222.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.300 - 2.900
R-factor	0.19544
Rwork	0.192
R-free	0.25658
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q5x
RMSD bond length	0.015
RMSD bond angle	1.590
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.560	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.120	0.510
Number of reflections	15208
<I/σ(I)>	10.2	2.8
Completeness [%]	99.6	99.5
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	CRYSTALS WERE PREPARED USING THE HANGING DROP METHOD AT 16 DEG. C BY MIXING 1:1 PROTEIN SAMPLE WITH MOTHER LIQUOR: 100 MM MAGNESIUM ACETATE, 100 MM MOPS PH 7.2 AND 12% W/V PEG 8000.THE CRYSTALS WERE TRANSFERRED BRIEFLY TO RESERVOIR SOLUTION SUPPLEMENTED WITH 25% (V/V) GLYCEROL AND FLASH FROZEN IN LIQUID NITROGEN.