2YJR
Structure of F1174L Mutant Anaplastic Lymphoma Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2010-12-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.439, 56.906, 102.201 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.100 - 1.900 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xp2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | CNX |
Refinement software | CNS (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.200 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.470 |
Number of reflections | 28564 | |
<I/σ(I)> | 20.8 | 4.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 286 | CRYSTALS WERE GROWN BY THE HANGING DROP VAPOR DIFFUSION METHOD AT 13 DEGREES CELSIUS BY MIXING 2 MICROLITERS OF PROTEIN SOLUTION WITH 2 MICROLITERS OF A RESERVOIR SOLUTION CONTAINING 22% (W/V) PEG 3350, 0.1 M TRIS PH 8.5, 0.2M LITHIUM SULFATE, 0.1% (W/V) BETA-OCTYLGLUCOSIDE. |