2YJP
Crystal structure of the solute receptors for L-cysteine of Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.710, 91.590, 158.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.180 - 2.260 |
| R-factor | 0.18713 |
| Rwork | 0.185 |
| R-free | 0.23274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xt8 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.946 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.180 | 2.380 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 39913 | |
| <I/σ(I)> | 11.32 | 2.25 |
| Completeness [%] | 97.8 | 86.7 |
| Redundancy | 4.14 | 2.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16-18% PEG 4000, 100 MM HEPES PH 7.0, 10 MM ZNCL2. |
