2YJN
Structure of the glycosyltransferase EryCIII from the erythromycin biosynthetic pathway, in complex with its activating partner, EryCII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-03-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 141.891, 141.891, 141.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.677 - 3.091 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.26150 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.862 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.100 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.040 | 0.270 |
| Number of reflections | 17457 | |
| <I/σ(I)> | 14 | 3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 MM PHOSPHATE, PH 8, 2MM DTT, 4M SODIUM FORMATE. |
