2YJL
Structural characterization of a secretin pilot protein from the type III secretion system (T3SS) of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.155, 48.358, 71.602 |
| Unit cell angles | 90.00, 96.92, 90.00 |
Refinement procedure
| Resolution | 35.540 - 1.810 |
| R-factor | 0.22138 |
| Rwork | 0.217 |
| R-free | 0.26224 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.920 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 35870 | |
| <I/σ(I)> | 29 | 3 |
| Completeness [%] | 90.0 | 75.6 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS 0.1 M PH 8.5, AMMONIUM SULFATE 1.5 M, GLYCEROL 10%. |
