2YJG
Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-16 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.410, 223.250, 46.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 114.960 - 1.800 |
R-factor | 0.18407 |
Rwork | 0.182 |
R-free | 0.22933 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MAD-SOLVED PARTIAL MODEL |
RMSD bond length | 0.020 |
RMSD bond angle | 1.729 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.000 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.330 |
Number of reflections | 73824 | |
<I/σ(I)> | 16 | 4.1 |
Completeness [%] | 97.0 | 91 |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP. RESERVOIR: 22%(W/V) PEGMME 5000, 0.2 M AMMONIUM SULFATE, 0.1 M MES PH 6.5, 3%(V/V) ETHYLENE GLYCOL. DROP: 1 MICROL PROTEIN AND 1 MICROL RESERVOIR. |