2YHK

D214A mutant of tyrosine phenol-lyase from Citrobacter freundii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-02
Detector	MARRESEARCH SX-165
Spacegroup name	P 21 21 2
Unit cell lengths	132.605, 142.705, 59.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.910
R-factor	0.15604
Rwork	0.156
R-free	0.17711
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2ez1
RMSD bond length	0.015
RMSD bond angle	1.393
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.970
High resolution limit [Å]	1.910	1.910
Rmerge	0.070	0.500
Number of reflections	87110
<I/σ(I)>	15	3.1
Completeness [%]	99.0	99
Redundancy	4.5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	THE CRYSTALS WERE GROWN AT 293 K BY THE HANGING DROP VAPOR DIFFUSION TECHNIQUE BY MIXING 2 UL OF THE PROTEIN SOLUTION, 18-20 MG/ML, WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION CONTAINING 50 MMOL/L TRIETHANOLAMINE BUFFER PH 8.0, 0.4-0.8 MOL/L KCL, 0.35-0.38 G/ML PEG 5000 MME, 5 MMOL/L PYRIDOXAL-5-PHOSPHATE, AND 2 MMOL/L DITHIOTHREITOL.