2YHK
D214A mutant of tyrosine phenol-lyase from Citrobacter freundii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-02 |
Detector | MARRESEARCH SX-165 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 132.605, 142.705, 59.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.910 |
R-factor | 0.15604 |
Rwork | 0.156 |
R-free | 0.17711 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2ez1 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.393 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.970 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 87110 | |
<I/σ(I)> | 15 | 3.1 |
Completeness [%] | 99.0 | 99 |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | THE CRYSTALS WERE GROWN AT 293 K BY THE HANGING DROP VAPOR DIFFUSION TECHNIQUE BY MIXING 2 UL OF THE PROTEIN SOLUTION, 18-20 MG/ML, WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION CONTAINING 50 MMOL/L TRIETHANOLAMINE BUFFER PH 8.0, 0.4-0.8 MOL/L KCL, 0.35-0.38 G/ML PEG 5000 MME, 5 MMOL/L PYRIDOXAL-5-PHOSPHATE, AND 2 MMOL/L DITHIOTHREITOL. |