2YHC
Structure of BamD from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.112, 33.428, 57.783 |
| Unit cell angles | 90.00, 111.41, 90.00 |
Refinement procedure
| Resolution | 28.390 - 1.800 |
| R-factor | 0.20007 |
| Rwork | 0.198 |
| R-free | 0.23262 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.095 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.510 |
| Number of reflections | 16896 | |
| <I/σ(I)> | 20.3 | 3.9 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5 . |
