2YH5
Structure of the C-terminal domain of BamC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.740, 59.120, 31.060 |
Unit cell angles | 90.00, 116.37, 90.00 |
Refinement procedure
Resolution | 29.560 - 1.250 |
R-factor | 0.14828 |
Rwork | 0.146 |
R-free | 0.18387 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.029 |
RMSD bond angle | 2.157 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.310 | 1.310 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.060 | 0.570 |
Number of reflections | 25297 | |
<I/σ(I)> | 10.3 | 2.1 |
Completeness [%] | 98.4 | 95.6 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG1000, 0.1 M MES PH 6.5 |