2YH3

The structure of BamB from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	101.841, 101.841, 110.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.21358
Rwork	0.211
R-free	0.26987
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.076
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.070	0.910
Number of reflections	17551
<I/σ(I)>	20.6	2.2
Completeness [%]	99.8	99.1
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		3.5M NH4CL, 0.1M NA-ACETATE PH 4.6