2YGX
Structure of the mixed-function P450 MycG in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.150, 161.980, 100.110 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 80.990 - 2.390 |
| R-factor | 0.18733 |
| Rwork | 0.184 |
| R-free | 0.24749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y98 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.715 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 161.980 | 2.520 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 68714 | |
| <I/σ(I)> | 10.8 | 3.2 |
| Completeness [%] | 95.6 | 76.6 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.6M SUCCINIC ACID PH 7, 2% GLYCEROL, 0.0075MM CYMAL-7 |
