2YGS
CARD DOMAIN FROM APAF-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.700, 36.000, 47.900 |
Unit cell angles | 90.00, 118.20, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.600 |
Rwork | 0.198 |
R-free | 0.23600 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.275 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.057 * | 0.120 * |
Total number of observations | 92435 * | |
Number of reflections | 11042 * | |
Completeness [%] | 96.8 * | 88.8 * |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * | 22 * | pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | acetate | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 24-28 (%(w/w)) | |
4 | 1 | reservoir | ammonium acetate | 200 (mM) | |
5 | 1 | reservoir | dithiothreitol | 10 (mM) |