2YGK
Crystal structure of the NurA nuclease from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.740, 80.280, 67.670 |
| Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
| Resolution | 35.940 - 2.500 |
| R-factor | 0.23382 |
| Rwork | 0.232 |
| R-free | 0.27089 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.940 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 1.330 |
| Number of reflections | 24087 | |
| <I/σ(I)> | 13.2 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M SODIUM CITRATE PH 4.5, 1 M LITHIUM CHLORIDE, 5% W/V PEG 6K, 50 MM MANGANESE CHLORIDE |
