2YGC
Structure of vaccinia virus D13 scaffolding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-14 |
Detector | ADSC CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 190.580, 190.580, 252.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.280 - 3.020 |
R-factor | 0.1717 |
Rwork | 0.170 |
R-free | 0.20290 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.300 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.210 | 1.500 |
Number of reflections | 53583 | |
<I/σ(I)> | 16.9 | 2 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 20.8 | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 4.0 M SODIUM FORMATE., pH 7.5 |