2YGA
E88G-N92L Mutant of N-Term HSP90 complexed with Geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.340, 74.340, 110.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.233 - 2.370 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MODEL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 13126 | |
| <I/σ(I)> | 9.5 | 1.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
