2YG2
Structure of apolioprotein M in complex with Sphingosine 1-Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.280, 68.280, 135.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.140 - 1.700 |
| R-factor | 0.19236 |
| Rwork | 0.191 |
| R-free | 0.22084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wew |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 40599 | |
| <I/σ(I)> | 15.5 | 3.34 |
| Completeness [%] | 98.9 | 98.3 |
| Redundancy | 6.07 | 6.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M LITHIUM SULFATE MONOHYDRATE, 0.1 M CITRIC ACID PH 3.5, 18 % W/V PEG6000 |
