2YFW
Heterotetramer structure of Kluyveromyces lactis Cse4,H4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-07-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | H 3 |
Unit cell lengths | 169.479, 169.479, 81.215 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.22112 |
Rwork | 0.218 |
R-free | 0.27633 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eqz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.120 | 0.810 |
Number of reflections | 26486 | |
<I/σ(I)> | 15 | 1.3 |
Completeness [%] | 99.9 | 98.8 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.1 M TRIS-HCL, PH8.5, 0.2 M NACL, AND 25% PEG 3350 |