2YFW
Heterotetramer structure of Kluyveromyces lactis Cse4,H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 |
| Unit cell lengths | 169.479, 169.479, 81.215 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.22112 |
| Rwork | 0.218 |
| R-free | 0.27633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.810 |
| Number of reflections | 26486 | |
| <I/σ(I)> | 15 | 1.3 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL, PH8.5, 0.2 M NACL, AND 25% PEG 3350 |
