2YFV
The heterotrimeric complex of Kluyveromyces lactis Scm3, Cse4 and H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 32.723, 65.530, 121.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.320 |
| R-factor | 0.22195 |
| Rwork | 0.220 |
| R-free | 0.25712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 11636 | |
| <I/σ(I)> | 17 | 1.5 |
| Completeness [%] | 94.7 | 55.3 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M SODIUM CITRATE, PH4.5, 6% PEG 4000, AND 0.1 M SODIUM IODIDE |
