2YFT
Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 171.204, 171.204, 115.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.890 - 1.850 |
| R-factor | 0.15701 |
| Rwork | 0.156 |
| R-free | 0.17801 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.890 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 72652 | |
| <I/σ(I)> | 24.6 | 5.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 14.1 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2 M (NH4)2SO4, 5% (V/V) 2-PROPANOL, pH 6 |
