2YFS
Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 171.951, 171.951, 116.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.450 - 2.600 |
| R-factor | 0.18196 |
| Rwork | 0.180 |
| R-free | 0.22397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 27086 | |
| <I/σ(I)> | 12.6 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2M (NH4)2)SO4, 5% (V/V) 2-PROPANOL, pH 6 |
