2YFN
galactosidase domain of alpha-galactosidase-sucrose kinase, AgaSK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.058, 111.650, 154.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.190 - 1.450 |
| R-factor | 0.13105 |
| Rwork | 0.130 |
| R-free | 0.14957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mi6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.447 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 158241 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 99.0 | 80.2 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 14% PEG 8000, 0.1M HEPES PH 7.5, PROTEIN CONCENTRATION 7MG/ML |
