2YFK
Crystal structure of a putative transcarbamoylase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 117.368, 117.368, 120.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.17083 |
| Rwork | 0.169 |
| R-free | 0.20162 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pvv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.913 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0112) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 30659 | |
| <I/σ(I)> | 8.1 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | LITHIUM SULFATE 180MM, PEG3350 24%, HEPES NA PH8 100MM, TRIS HCL PH7.5 40MM |
