2YF4
Crystal structure of DR2231, the MazG-like protein from Deinococcus radiodurans, Apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.778, 110.828, 166.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.20993 |
| Rwork | 0.209 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yeu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.389 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.130 |
| Number of reflections | 137940 | |
| <I/σ(I)> | 16 | 7.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.01M MAGNESIUM CHLORIDE,0.05M SODIUM CACODYLATE, PH 6.0, 1M LITHIUM SULFATE |
