2YF2
Crystal structure of the oligomerisation domain of C4b-binding protein from Gallus gallus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.888, 140.238, 85.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.060 - 2.240 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.23570 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.460 | 2.360 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 23669 | |
| <I/σ(I)> | 13.5 | 2.2 |
| Completeness [%] | 96.0 | 79.6 |
| Redundancy | 4.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M AMMONIUM ACETATE, 0.1M SODIUM ACETATE, 8% PEG 3350, PH 4.5 |
