2YES
Crystal Structure of Rv0371C complex with Manganese from Mycobacterium Tuberculosis H37Rv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-16 |
| Detector | BRUKER PROTEUM 300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.220, 59.697, 146.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.900 |
| R-factor | 0.20507 |
| Rwork | 0.203 |
| R-free | 0.23533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wee |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.845 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 30184 | |
| <I/σ(I)> | 31.27 | 3.76 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% (V/V) PEG 4000, 0.1 M MES (PH 6.0) BY MICROSEEDING |
