2YDY
Crystal structure of human S-adenosylmethionine synthetase 2, beta subunit in Orthorhombic crystal form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.980, 111.770, 123.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.070 - 2.250 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21750 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.610 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.050 | 0.910 |
| Number of reflections | 15419 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
