2YDT

STRUCTURE OF THE ALPHA-L-ARABINOFURANOSIDASE ARB93A from FUSARIUM Graminearum in complexe with an iminosugar inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-16
Detector	ADSC CCD
Spacegroup name	I 41 2 2
Unit cell lengths	105.435, 105.435, 140.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.690 - 1.600
R-factor	0.144
Rwork	0.142
R-free	0.17000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w5n
RMSD bond length	0.016
RMSD bond angle	1.604
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.690	1.670
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.380
Number of reflections	55107
<I/σ(I)>	11	2
Completeness [%]	100.0	99.8
Redundancy	7.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		38% PEG 550MME, 100 MM HEPES PH 7.5, 100 MM MNSO4.