2YDT
STRUCTURE OF THE ALPHA-L-ARABINOFURANOSIDASE ARB93A from FUSARIUM Graminearum in complexe with an iminosugar inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 105.435, 105.435, 140.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.690 - 1.600 |
| R-factor | 0.144 |
| Rwork | 0.142 |
| R-free | 0.17000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w5n |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.604 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 55107 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 38% PEG 550MME, 100 MM HEPES PH 7.5, 100 MM MNSO4. |
