2YDD
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, soaked with 2',3'-cyclic AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-14 |
Detector | MARRESEARCH MAR165 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.360, 46.810, 106.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.290 - 2.400 |
R-factor | 0.21019 |
Rwork | 0.207 |
R-free | 0.26943 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.258 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.940 |
Number of reflections | 8420 | |
<I/σ(I)> | 13 | 1.8 |
Completeness [%] | 98.2 | 99.5 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.5 | CRYSTALLIZATION: 50 MM NA-CITRATE PH 3.5 & 30 % PEG 3000, SOAKING: 50 MM NA-CITRATE PH 3.5, 35 % PEG 1500 & 100 MM 2,3-CYCLIC AMP |