2YDD

Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, soaked with 2',3'-cyclic AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-14
Detector	MARRESEARCH MAR165
Spacegroup name	P 21 21 21
Unit cell lengths	41.360, 46.810, 106.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.290 - 2.400
R-factor	0.21019
Rwork	0.207
R-free	0.26943
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmi
RMSD bond length	0.010
RMSD bond angle	1.258
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.940
Number of reflections	8420
<I/σ(I)>	13	1.8
Completeness [%]	98.2	99.5
Redundancy	4.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		3.5		CRYSTALLIZATION: 50 MM NA-CITRATE PH 3.5 & 30 % PEG 3000, SOAKING: 50 MM NA-CITRATE PH 3.5, 35 % PEG 1500 & 100 MM 2,3-CYCLIC AMP