2YDC

Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, soaked with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-15
Detector	MARRESEARCH MAR165
Spacegroup name	P 21 21 21
Unit cell lengths	41.890, 46.430, 106.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.946 - 2.050
R-factor	0.2041
Rwork	0.202
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmi
RMSD bond length	0.003
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.080	1.080
Number of reflections	13618
<I/σ(I)>	15.66	2.18
Completeness [%]	99.9	100
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		3.5		CRYSTALLIZATION: 50 MM NA-CITRATE PH 3.5 & 30 % PEG 3000, SOAKING: 50 MM NA-CITRATE PH 3.5, 35 % PEG 1500 & 20 MM GTP FOR 3 H