2YD7
Crystal structure of the N-terminal Ig1-2 module of Human Receptor Protein Tyrosine Phosphatase Delta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.157, 31.724, 92.323 |
| Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
| Resolution | 85.610 - 1.980 |
| R-factor | 0.22029 |
| Rwork | 0.219 |
| R-free | 0.24881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 30633 | |
| <I/σ(I)> | 16.8 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% W/V PEG 3350, 0.2M AMMONIUM DI-HYDROGEN PHOSPHATE . |
