2YD1
Crystal structure of the N-terminal Ig1-2 module of Drosophila Receptor Protein Tyrosine Phosphatase DLAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.201, 29.074, 49.408 |
| Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
| Resolution | 47.175 - 1.800 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.530 |
| Number of reflections | 16271 | |
| <I/σ(I)> | 19.6 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 25% W/V PEG 1500, 0.1M SPG SYSTEM, 35MM AMMONIUM SULPHATE, PH 5.0 |
