2YCL

complete structure of the corrinoid,iron-sulfur protein including the N-terminal domain with a 4Fe-4S cluster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	172.219, 43.476, 101.855
Unit cell angles	90.00, 119.39, 90.00

Refinement procedure

Resolution	33.745 - 1.950
R-factor	0.1946
Rwork	0.192
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h9a
RMSD bond length	0.012
RMSD bond angle	1.647
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.100	0.530
Number of reflections	48286
<I/σ(I)>	10.36	2.55
Completeness [%]	99.3	99.4
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1