2YCH
PilM-PilN type IV pilus biogenesis complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.780, 50.780, 366.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.300 - 2.200 |
| R-factor | 0.23947 |
| Rwork | 0.237 |
| R-free | 0.27944 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.341 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD (2006) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.000 | 2.210 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 27534 | |
| <I/σ(I)> | 16.3 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M TRIS PH 8.0, 0.2M MAGNESIUM CHLORIDE AND 20% (W/V) PEG 6000 |
