2YBD

Crystal structure of probable had family hydrolase from pseudomonas fluorescens pf-5 with bound phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU-MSC RAXIS IV
Spacegroup name	P 61 2 2
Unit cell lengths	65.978, 65.978, 250.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.989 - 2.001
R-factor	0.173
Rwork	0.171
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m9l
RMSD bond length	0.008
RMSD bond angle	1.032
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.300
Number of reflections	22605
<I/σ(I)>	32.6	6.4
Completeness [%]	98.5	89.2
Redundancy	15.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		PROTEIN (10MM HEPES PH 7.5, 150 MM NACL, 10% GLYCEROL, 5 MM DTT, 5 MM MGCL);RESERVOUR (2.5 M NA/K PHOSPHATE, 150 MM NACITRATE, PH 5.0;CRYOPROTECTION (1.8 M NA/K PHOSPHATE, 100 MM NACITRATE, 18.5% GLYCEROL, 185 MM MGSO4) TWO MINUTE SOAK.