2YB7
CBM62 in complex with 6-alpha-D-Galactosyl-mannotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 191.352, 191.352, 191.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.430 - 1.700 |
| R-factor | 0.16607 |
| Rwork | 0.165 |
| R-free | 0.18565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y9S |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.578 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.400 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.150 | 0.900 |
| Number of reflections | 60268 | |
| <I/σ(I)> | 14 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.5 M AMMONIUM SULPHATE IN 0.1 M NA/HEPES, PH 7.4, CONTAINING 30 % 2-METHYL-2,4-PENTANEDIOL. |
