2YB4

Structure of an amidohydrolase from Chromobacterium violaceum (EFI target EFI-500202) with bound SO4, no metal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	40.070, 46.530, 130.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.775 - 2.200
R-factor	0.1735
Rwork	0.170
R-free	0.23270
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.053
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.260
Number of reflections	12953
<I/σ(I)>	10.3	4.8
Completeness [%]	99.7	100
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.1		PROTEIN (15 MG/ML IN 10 MM HEPES PH 7.5, 150 MM NACL, 10% GLYCEROL) ADDED TO 25% PEG 3350, 100 MM BIS-TRIS PH 6.1, 200 MM AMSO4. CRYOSOLUTION WAS RESERVOIR WITH 20% ETHYLENE GLYCOL