2YA1
Product complex of a multi-modular glycogen-degrading pneumococcal virulence factor SpuA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.137, 86.429, 193.225 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.2082 |
Rwork | 0.205 |
R-free | 0.26519 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fh6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.348 |
Data reduction software | CrystalClear |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.090 | 0.400 |
Number of reflections | 56573 | |
<I/σ(I)> | 9.4 | 2.8 |
Completeness [%] | 92.6 | 94.8 |
Redundancy | 4.25 | 4.18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |