2Y9Z
Chromatin Remodeling Factor ISW1a(del_ATPase) in DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-18 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 284.028, 284.028, 193.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.955 - 3.601 |
| R-factor | 0.2833 |
| Rwork | 0.283 |
| R-free | 0.29140 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.703 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | HKL2MAP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.670 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 53279 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 50MM BISTRIS(PH7.0), 150MM NACITRATE, 30%(W/V) PEG5000 |
