2Y9M
Pex4p-Pex22p structure
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.328, 43.194, 60.474 |
| Unit cell angles | 90.00, 100.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.20751 |
| Rwork | 0.205 |
| R-free | 0.25924 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 11039 | |
| <I/σ(I)> | 26.7 | 4.8 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 8.0 % (V/V) ETHYLENE GLYCOL, 14.0 % (W/V) POLYETHYLENE GLYCOL, 0.1 M HEPES PH 7.0 |
